Ureaplasma parvum e Ureaplasma urealyticum em amostras cervicais de mulheres com candidíase vulvovaginal e com vaginose bacteriana
DOI:
https://doi.org/10.53660/CONJ-742-B18Palavras-chave:
Ureaplasma parvum, Ureaplasma urealyticum, Candidíase vulvovaginal, Vaginose bacterianaResumo
A microbiota vaginal tem uma importância fundamental na saúde do trato genital feminino. A presença de Ureaplasma spp. tem sido relatada com frequência em estudos de correlação com o Papilomavírus Humano (HPV). Objetivou-se determinar a prevalência de Ureaplasma parvum (UP) e Ureaplasma urealyticum (UU) em mulheres com Vaginose Bacteriana (VB) e Candidíase Vulvovaginal (CVV) e correlacionar com HPV. Extraiu-se o DNA genômico de 64 amostras cervicais, utilizando o kit comercial QIAamp® DNA Mini Kit. Identificou-se UP e UU por Multiplex PCR convencional e para HPV utilizou-se Nested PCR/ sequenciamento. Verificou-se que a prevalência de UP no grupo de CVV foi de 87,8% (29/33) e no de VB foi de 96,7% (30/31). O UU demonstrou prevalência de 81,8% (27/33) nas amostras de CVV e 67,7% (21/33) nas de VB. Quanto ao HPV, 27,2% (9/33) das amostras de CVV foram positivas para a presença deste patógeno e nas amostras de VB, 35,4% (11/31) das amostras também foram positivas. Observou-se que existe uma tendência de infecção pelo HPV na presença de Ureaplasma spp. em especial o UP. Todavia, tal afirmação não pode ser confirmada neste trabalho por não demonstrar significância estatística.
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